Difference between revisions of "Part:BBa K1680009"
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This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by  Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.
 
This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by  Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.
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[[Image:PSUC-conc.png|400px]]
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RLU measurement of the NanoLuc under pSUC2 promoter [[BBa_K950003]] control, normalized to OD600.
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[[Image:Team Tuebingen mpr 6h.jpg|400px]]
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RLU measurement of the NanoLuc under pFET3 promoter [[BBa_K950000]] control, normalized to OD600.
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Revision as of 00:25, 26 September 2015

NanoLuc Luciferase

This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.


PSUC-conc.png RLU measurement of the NanoLuc under pSUC2 promoter BBa_K950003 control, normalized to OD600.

Team Tuebingen mpr 6h.jpg RLU measurement of the NanoLuc under pFET3 promoter BBa_K950000 control, normalized to OD600.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]