Difference between revisions of "Part:BBa K1680013:Design"
 
Line 2: Line 2:
 
<partinfo>BBa_K1680013 short</partinfo>
 
<partinfo>BBa_K1680013 short</partinfo>
  
−
 
+
Contains a NLS (Nuclear localisation sequence) followed by a spacer and a modified TEV-cleavage site. After Protease cleavage Phenylalanine is exposed N-terminally, which leads to rapid protein depletion. In addition to that, after cleavage a NES (Nuclear export sequence) is exposed.
  
 
<partinfo>BBa_K1680013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1680013 SequenceAndFeatures</partinfo>
Line 8: Line 8:
  
 
===Design Notes===
 
===Design Notes===
−
Shipped in RFC10
+
Shipped in RFC25
  
  

Latest revision as of 00:24, 26 September 2015

NDegron

Contains a NLS (Nuclear localisation sequence) followed by a spacer and a modified TEV-cleavage site. After Protease cleavage Phenylalanine is exposed N-terminally, which leads to rapid protein depletion. In addition to that, after cleavage a NES (Nuclear export sequence) is exposed.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Shipped in RFC25


Source

gBlock designed by Team Tuebingen

References

Taxis, Christof, et al. "Efficient protein depletion by genetically controlled deprotection of a dormant N‐degron." Molecular systems biology 5.1 (2009): 267.