Difference between revisions of "Part:BBa K1680013"
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This part contains the protein coding region for an NDegron as described by Taxis et al. 2009. The NDegron enhances protein turnover by destabilising the protein it is fused to upon cleavage by TEV protease. | This part contains the protein coding region for an NDegron as described by Taxis et al. 2009. The NDegron enhances protein turnover by destabilising the protein it is fused to upon cleavage by TEV protease. | ||
The part is supplied in RFC25. This part was intended as a backup for our project, due to time restraints we did not use it. | The part is supplied in RFC25. This part was intended as a backup for our project, due to time restraints we did not use it. | ||
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Latest revision as of 00:05, 26 September 2015
NDegron
This part contains the protein coding region for an NDegron as described by Taxis et al. 2009. The NDegron enhances protein turnover by destabilising the protein it is fused to upon cleavage by TEV protease. The part is supplied in RFC25. This part was intended as a backup for our project, due to time restraints we did not use it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 157 - 1000COMPATIBLE WITH RFC[1000]