Difference between revisions of "Part:BBa K563004:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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pTEF1 is also listed as parts BBa_K431008 and BBa_K319003. | pTEF1 is also listed as parts BBa_K431008 and BBa_K319003. | ||
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| + | <partinfo>BBa_K563004 AddReview 4</partinfo> | ||
| + | <I>Chalmers 2015</I> | ||
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| + | Additional improvement added by | ||
| + | Author: John Hellgren | ||
| + | Summary: The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. [https://parts.igem.org/Part:BBa_K1603001 pTEF1-pSUC2] | ||
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Revision as of 20:53, 25 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K563004
User Reviews
UNIQ67551981469b691b-partinfo-00000000-QINU UNIQ67551981469b691b-partinfo-00000001-QINU
UNIQ67551981469b691b-partinfo-00000002-QINU
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UCSF-UCB iGEM 2014 |
We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.
The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275. pTEF1 is also listed as parts BBa_K431008 and BBa_K319003.
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Chalmers 2015 |
Additional improvement added by Author: John Hellgren Summary: The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. pTEF1-pSUC2
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