Difference between revisions of "Part:BBa K1676103"
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This part is the Lactate Permease gene of E. Coli. It's function is to transport lactate into the cytoplasm. Previous studies have compared it's efficiency when cloned into Shewanella oneidensis MR1. However, it was found that the homolog from Pseudomonas aeruginosa enhances lactate consumption and growth of our chasis more than this gene. | This part is the Lactate Permease gene of E. Coli. It's function is to transport lactate into the cytoplasm. Previous studies have compared it's efficiency when cloned into Shewanella oneidensis MR1. However, it was found that the homolog from Pseudomonas aeruginosa enhances lactate consumption and growth of our chasis more than this gene. | ||
| + | More information on the characterisation of this gene can be found in this summary. Guides on the legend can be found at the last page. | ||
https://static.igem.org/mediawiki/2015/5/58/Prescreening_of_enzymes.pdf | https://static.igem.org/mediawiki/2015/5/58/Prescreening_of_enzymes.pdf | ||
Revision as of 18:34, 25 September 2015
Lactate Permease of E. Coli
This part is the Lactate Permease gene of E. Coli. It's function is to transport lactate into the cytoplasm. Previous studies have compared it's efficiency when cloned into Shewanella oneidensis MR1. However, it was found that the homolog from Pseudomonas aeruginosa enhances lactate consumption and growth of our chasis more than this gene.
More information on the characterisation of this gene can be found in this summary. Guides on the legend can be found at the last page.
https://static.igem.org/mediawiki/2015/5/58/Prescreening_of_enzymes.pdf
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 48
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]