Difference between revisions of "Part:BBa J63009:Design"

 
(→‎Design Notes: added link to design notes on silver lab on oww)
 
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If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.
 
If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.
  
 +
from [http://openwetware.org/wiki/Silver:_Commonly_Used_Plasmids Silver: Commonly Used Plasmids] on OpenWetWare:
 +
* "We commonly use two Biobrick vectors: V0120 (same as V0100) and V0002. The V0120 vector preferred over the V0002 vector because V0120 is a high-copy plasmid (~300 ng/uL from a mini-prep of a 5 mL culture) while V0002 is not. The V0002 plasmid is most frequently found in older parts."
  
 +
* "The annonated sequence of V0002 can be found [http://openwetware.org/images/5/5c/BBa_V0120.gb here]"
  
 
===Source===
 
===Source===

Latest revision as of 04:26, 2 May 2007


Low copy protein fusion vector (Silver lab standard)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2057
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2057
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1096


Design Notes

If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.

from [http://openwetware.org/wiki/Silver:_Commonly_Used_Plasmids Silver: Commonly Used Plasmids] on OpenWetWare:

  • "We commonly use two Biobrick vectors: V0120 (same as V0100) and V0002. The V0120 vector preferred over the V0002 vector because V0120 is a high-copy plasmid (~300 ng/uL from a mini-prep of a 5 mL culture) while V0002 is not. The V0002 plasmid is most frequently found in older parts."
  • "The annonated sequence of V0002 can be found [http://openwetware.org/images/5/5c/BBa_V0120.gb here]"

Source

based on pUC19 plasmid

References