Difference between revisions of "Part:BBa K1859027:Experience"

 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K1859027===
 
  
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===Applications of BBa_K1859027===
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We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 
We inserted this generator into <i>E. coli</i> K-12 JM109.<br>

Latest revision as of 05:15, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Applications of BBa_K1859027



We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane C shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.


User Reviews

UNIQ9e6ec206f97ea89a-partinfo-00000001-QINU UNIQ9e6ec206f97ea89a-partinfo-00000002-QINU