Difference between revisions of "Part:BBa K1616023"
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Monomeric enzyme | Monomeric enzyme | ||
NanoLuc facilitates use as transcriptional reporter or fusion partner. | NanoLuc facilitates use as transcriptional reporter or fusion partner. | ||
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<center> | <center> | ||
[[Image:Nanoluc reaction copie.png|600px|]] | [[Image:Nanoluc reaction copie.png|600px|]] | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. | |
===Source=== | ===Source=== | ||
Revision as of 01:14, 23 September 2015
T7 promoter - HisTag- NanoLuc - T7 terminator
NanoLuc (Nluc) is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter.
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
Thermal stable enzyme
NanoLuc retains activity following 30 min incubation at 55 °C.
Active over broad pH range
NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7.
Monomeric enzyme
NanoLuc facilitates use as transcriptional reporter or fusion partner.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
Design Notes
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
Source
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
References
Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012
Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000.