Difference between revisions of "Part:BBa K1616005:Experience"

Revision as of 00:03, 23 September 2015

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Applications of BBa_K1616005

Notebook

Ligation of Gblocks Holin-Endolysin into pDawn

Tube	Water	T4 ligase Buffer	pDawn	Plasmid	T4 ligase
Holin - Ensolysin	4,6 µL	1 µL	1,4 µL	2,5 µL	0,5 µL

Results

Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.

In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.

User Reviews

UNIQb59b210faf263cc6-partinfo-00000000-QINU UNIQb59b210faf263cc6-partinfo-00000001-QINU

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 					</tr>
-				<tr><td><b>Holin - Ensolysin </b></td>
+				<tr><td><font style="color:#b22222">Holin - Ensolysin </font></td>
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 							<center>4,6 µL</center>