Difference between revisions of "Part:BBa K1616005:Experience"
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</th> | </th> | ||
<th> | <th> | ||
| − | + | Water | |
| + | </th> | ||
| + | <th> | ||
| + | T4 ligase Buffer | ||
| + | </th> | ||
| + | <th> | ||
| + | pDawn | ||
| + | </th> | ||
| + | <th> | ||
| + | Plasmid | ||
| + | </th><th> | ||
| + | T4 ligase | ||
</th> | </th> | ||
</tr> | </tr> | ||
| − | <tr> | + | <tr><td><b>Holin - Ensolysin </b></td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td> | <td> | ||
4,6 µL | 4,6 µL | ||
</td> | </td> | ||
| − | + | <td> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
1 µL | 1 µL | ||
</td> | </td> | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td> | <td> | ||
1,4 µL | 1,4 µL | ||
</td> | </td> | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td> | <td> | ||
2,5 µL | 2,5 µL | ||
</td> | </td> | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td> | <td> | ||
Revision as of 00:00, 23 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1616005
Notebook
Ligation of Gblocks Holin-Endolysin into pDawn
|
Tube |
Water |
T4 ligase Buffer |
pDawn |
Plasmid |
T4 ligase |
|---|---|---|---|---|---|
| Holin - Ensolysin |
4,6 µL |
1 µL |
1,4 µL |
2,5 µL |
0,5 µL |
Results
Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.
In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.
User Reviews
UNIQbb94fdf8976ff06a-partinfo-00000000-QINU UNIQbb94fdf8976ff06a-partinfo-00000001-QINU