Difference between revisions of "Part:BBa K1616005:Experience"
(→‎Applications of BBa_K1616005)
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===Applications of BBa_K1616005===
 
===Applications of BBa_K1616005===
  
−
<h2><font style="color:#b22222"> Notebook </font></h2>
+
<h2> Notebook </h2>
  
 +
<h3><font style="color:#b22222">Ligation of Gblocks Holin-Endolysin into pDawn </font></h3>
  
 +
<table id="Mixligation">
 +
 +
<tbody><tr>
 +
<th>
 +
Tube
 +
</th>
 +
<th>
 +
H/E
 +
</th>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<b>Water</b>
 +
</td>
 +
 +
<td>
 +
4,6 µL
 +
</td>
 +
 +
</tr><tr>
 +
<td>
 +
<b>T4 ligase Buffer</b>
 +
</td>
 +
 +
 +
<td>
 +
1 µL
 +
</td>
 +
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
<b>pDawn</b>
 +
</td>
 +
 +
<td>
 +
1,4 µL
 +
</td>
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
<b>Plasmid</b>
 +
</td>
 +
 +
<td>
 +
2,5 µL
 +
</td>
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
T4 ligase
 +
</td>
 +
 +
<td>
 +
0,5 µL
 +
</td>
 +
 +
</tr>
 +
</tbody></table>
  
−
<h2><font style="color:#b22222"> Results </font></h2>
+
<h2>Results </h2>
  
 
<center>[[Image:Graph-HE.png|center|600px|]] </center>
 
<center>[[Image:Graph-HE.png|center|600px|]] </center>

Revision as of 23:56, 22 September 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_K1616005

Notebook

Ligation of Gblocks Holin-Endolysin into pDawn

<tbody> </tbody>

Tube

H/E

Water

4,6 µL

T4 ligase Buffer

1 µL

pDawn

1,4 µL

Plasmid

2,5 µL

T4 ligase

0,5 µL

Results

Graph-HE.png


Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.

In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.

User Reviews

UNIQ8cf3217f8977ceb7-partinfo-00000000-QINU UNIQ8cf3217f8977ceb7-partinfo-00000001-QINU