Difference between revisions of "Part:BBa K1618021"

Revision as of 17:19, 22 September 2015

Plac-THL-CRO-BHBD-BCD-EFTb-EFTa

K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal NheI site found at 1167
Illegal NotI site found at 235
Illegal NotI site found at 3841
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal BglII site found at 1415
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
Illegal XbaI site found at 244
Illegal AgeI site found at 1338
Illegal AgeI site found at 3354
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1618021 short</partinfo>
-Acetate to Butyrate pathway with IPTG inducible Promoter
+K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA.
+[[File:NRP-UEA-Norwich-Flavs-Thing.png]]
+<i>Figure 1: The butyrate biosynthetic pathway. </i>
 <!-- Add more about the biology of this part here