Difference between revisions of "Part:BBa K1806005:Experience"
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The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. The part was tagged as G-Block 7 and sample 7-6 yielded the accurate base pair length. | The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. The part was tagged as G-Block 7 and sample 7-6 yielded the accurate base pair length. | ||
| − | [[File:6005-1.png]] [[File: | + | [[File:6005-1.png]] [[File:6105-2.png]] |
[[File:AUC TURKEY 7,6 cutcheck.png]] [[File:6005-4.png]] | [[File:AUC TURKEY 7,6 cutcheck.png]] [[File:6005-4.png]] | ||
Revision as of 10:21, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1806005
Cloning
The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. The part was tagged as G-Block 7 and sample 7-6 yielded the accurate base pair length.
User Reviews
UNIQ75cd706b02d548e4-partinfo-00000000-QINU UNIQ75cd706b02d548e4-partinfo-00000001-QINU