Difference between revisions of "Part:BBa K1859023:Experience"
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===Applications of BBa_K1859023=== | ===Applications of BBa_K1859023=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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| + | <html> | ||
| + | <p> | ||
| + | <a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015] | ||
| + | </a> . | ||
| + | </p> | ||
| + | <p> | ||
| + | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
| + | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
| + | Lane H shows this sample.</p><br><br> | ||
| + | |||
| + | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | ||
| + | <br><br> | ||
| + | <p>There was no fluorescence. We found that this generator doesn’t make functional long chain protein.</p> | ||
| + | |||
| + | </html> | ||
Revision as of 10:11, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859023
User Reviews
UNIQ52cf9c1d9fa022f4-partinfo-00000000-QINU UNIQ52cf9c1d9fa022f4-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane H shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.