Difference between revisions of "Part:BBa K1859022:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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===Applications of BBa_K1859022=== | ===Applications of BBa_K1859022=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1859022 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1859022 EndReviews</partinfo> | ||
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| + | <html> | ||
| + | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
| + | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
| + | Lane G shows this sample.<br><br> | ||
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| + | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | ||
| + | <br><br> | ||
| + | There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br> | ||
| + | |||
| + | </html> | ||
Revision as of 10:07, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859022
User Reviews
UNIQ4ff8d8115b2af301-partinfo-00000000-QINU UNIQ4ff8d8115b2af301-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.