Difference between revisions of "Part:BBa K1859020:Experience"

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<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
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We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 
We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 
We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
 
We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
 
Lane E shows this sample.<br><br>
 
Lane E shows this sample.<br><br>
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<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img>
 
<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img>
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<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
 
<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
 
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<br><br>
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br>
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There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.</p>
 
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Revision as of 09:42, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

User Reviews

UNIQ45c2c8c4f4fe48ea-partinfo-00000000-QINU UNIQ45c2c8c4f4fe48ea-partinfo-00000001-QINU

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.