Difference between revisions of "Part:BBa K1859024"
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| − | We found that circular efficiency of [ | + | We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators |
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [ | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a> |
, [BBa_1859024], | , [BBa_1859024], | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a> | ||
Latest revision as of 09:41, 22 September 2015
mRNA circularize efficiently generator (inside complementarityⅠ)
We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.
We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .