Difference between revisions of "Part:BBa K1859020:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | ===Applications of BBa_K1859020=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
Revision as of 09:35, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859020
User Reviews
UNIQae32925ade96e7f3-partinfo-00000000-QINU UNIQae32925ade96e7f3-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.