Difference between revisions of "Part:BBa K1859027:Experience"
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We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
| − | Lane | + | Lane C shows this sample.<br><br> |
<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
Revision as of 09:27, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
===Applications of BBa_K1859027===
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane C shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.
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