Difference between revisions of "Part:BBa K1859023:Experience"

Revision as of 09:23, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859023===

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane H shows this sample.

left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.

User Reviews

UNIQf2b40c5f6f3c03c0-partinfo-00000001-QINU UNIQf2b40c5f6f3c03c0-partinfo-00000002-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
+<html>
 ===Applications of BBa_K1859023===
+<br><br>
+We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
+We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
+Lane H shows this sample.<br><br>
+<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img>
+<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img>
+<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img>
+<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
+<br><br>
+There was no fluorescence. We found that this generator doesn’t make functional long chain protein.<br>
+</html>
 ===User Reviews===