Difference between revisions of "Part:BBa K1859022:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| + | |||
| + | <html> | ||
===Applications of BBa_K1859022=== | ===Applications of BBa_K1859022=== | ||
| + | |||
| + | <br><br> | ||
| + | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
| + | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
| + | Lane G shows this sample.<br><br> | ||
| + | |||
| + | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | ||
| + | <br><br> | ||
| + | There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br> | ||
| + | |||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:20, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
===Applications of BBa_K1859022===
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.
===User Reviews===