Difference between revisions of "Part:BBa K1859020:Experience"

Revision as of 08:40, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.

Fluorescence of protein before dying by CBB Fig.5 after dying by CBB

</b></p> <img src="" width="" height="160" vspace="10" hspace="50" align="left"></img>

Fig.6 Overlapping Fig.4 and Fig.5
There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.

User Reviews

UNIQc67c040cb8c377ba-partinfo-00000000-QINU UNIQc67c040cb8c377ba-partinfo-00000001-QINU

@@ Line 8: / Line 8: @@
 We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
- Lane E shows this sample.<br>
+Lane E shows this sample.<br>
 <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="111" height="160" vspace="10" hspace="50" align="left"></img>
@@ Line 17: / Line 17: @@
 <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="160" vspace="10" hspace="50" align="left"></img>
-<br clear="all">
-<b>Fig.6 Overlapping Fig.4 and Fig.5 </b><br><br>
+<b>Fig.6 Overlapping Fig.4 and Fig.5 </b><br>
 &nbsp;&nbsp;There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.