Difference between revisions of "Part:BBa K1859020:Experience"

 
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===Applications of BBa_K1859020===
 
===Applications of BBa_K1859020===
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We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
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We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
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Lane E shows this sample.
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<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="222" height="320" vspace="10" hspace="50" align="left"></img>
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<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="292" height="320" vspace="10" hspace="50" align="right"></img>
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<br clear="all">
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<p><b>Fig.4 Fluorescence of protein before dying by CBB</b><b>  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;     
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Fig.5 after dying by CBB<br><br></b></p>
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<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="304" height="357" vspace="10" hspace="50" align="left"></img>
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<br clear="all">
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<b>Fig.6 Overlapping Fig.4 and Fig.5 </b><br><br>
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&nbsp;&nbsp;There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:36, 22 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.

Lane E shows this sample.

<img src="Gifu-sds-before.jpeg" width="222" height="320" vspace="10" hspace="50" align="left"></img> <img src="Gifu-sds-dye.jpg" width="292" height="320" vspace="10" hspace="50" align="right"></img>


Fig.4 Fluorescence of protein before dying by CBB                                                          Fig.5 after dying by CBB

<img src="Gifu-sds-page-combine.png" width="304" height="357" vspace="10" hspace="50" align="left"></img>


Fig.6 Overlapping Fig.4 and Fig.5

  There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.

User Reviews

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