Difference between revisions of "Part:BBa K1582029"
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<partinfo>BBa_K1582029 parameters</partinfo> | <partinfo>BBa_K1582029 parameters</partinfo> | ||
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| + | ===Usage and Biology=== | ||
| + | This year, we focus on solving the protein purification problem in biological lab. As we talked about in the Protein Extraction Kit page in project discription, we aim to replace the lengthy and expensive chromatography with Janus-based aquous two-phase system. <br> | ||
| + | |||
| + | Above all, we take advantage of synthetic biology, using the core of iGEM- BioBrick and RFC Assembly Method, to build a universal purification tag. This tag includes a linker containing TEV protease site and sJanus, designed by the RFC23 method which is specific for fusion protein. Thus, if other teams want to use this system to express and purify their proteins, they could use the standard assembly to make a fusion protein. <br> | ||
Latest revision as of 04:44, 22 September 2015
Protein Extraction Tag: Linker+sJanus
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 214
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 223
Usage and Biology
This year, we focus on solving the protein purification problem in biological lab. As we talked about in the Protein Extraction Kit page in project discription, we aim to replace the lengthy and expensive chromatography with Janus-based aquous two-phase system.
Above all, we take advantage of synthetic biology, using the core of iGEM- BioBrick and RFC Assembly Method, to build a universal purification tag. This tag includes a linker containing TEV protease site and sJanus, designed by the RFC23 method which is specific for fusion protein. Thus, if other teams want to use this system to express and purify their proteins, they could use the standard assembly to make a fusion protein.