Difference between revisions of "Part:BBa K1321357:Experience"
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===Applications of BBa_K1321357=== | ===Applications of BBa_K1321357=== | ||
We improved and expanded characterisation on the part BBa_K1321357, a cellulose binding domain (CBD) submitted by the Imperial College 2014 team. After confirming expression through SDS PAGE we incubated iso-standard paper chads from Whatman 54 filter paper in a crude cell lysate containing the CBD bound to super folded GFP for ten minutes. We then let the protein saturated chads sit in a large volume of 1 x PBS buffer at pH 7.4 (to remain consistent with Imperial’s initial characterisation). At different time points chads were taken from the buffer and placed into a 96 well plate, over 30 minutes. The decrease in fluorescence over time was indicative of relative binding affinity, to Whatman 54 paper. The graph below shows our results. | We improved and expanded characterisation on the part BBa_K1321357, a cellulose binding domain (CBD) submitted by the Imperial College 2014 team. After confirming expression through SDS PAGE we incubated iso-standard paper chads from Whatman 54 filter paper in a crude cell lysate containing the CBD bound to super folded GFP for ten minutes. We then let the protein saturated chads sit in a large volume of 1 x PBS buffer at pH 7.4 (to remain consistent with Imperial’s initial characterisation). At different time points chads were taken from the buffer and placed into a 96 well plate, over 30 minutes. The decrease in fluorescence over time was indicative of relative binding affinity, to Whatman 54 paper. The graph below shows our results. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:14, 22 September 2015
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Applications of BBa_K1321357
We improved and expanded characterisation on the part BBa_K1321357, a cellulose binding domain (CBD) submitted by the Imperial College 2014 team. After confirming expression through SDS PAGE we incubated iso-standard paper chads from Whatman 54 filter paper in a crude cell lysate containing the CBD bound to super folded GFP for ten minutes. We then let the protein saturated chads sit in a large volume of 1 x PBS buffer at pH 7.4 (to remain consistent with Imperial’s initial characterisation). At different time points chads were taken from the buffer and placed into a 96 well plate, over 30 minutes. The decrease in fluorescence over time was indicative of relative binding affinity, to Whatman 54 paper. The graph below shows our results.
User Reviews
UNIQf8e46b7bf9bb20d7-partinfo-00000000-QINU UNIQf8e46b7bf9bb20d7-partinfo-00000001-QINU