Difference between revisions of "Part:BBa K1615101"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1615101 short</partinfo>
 
<partinfo>BBa_K1615101 short</partinfo>
  
−
mRFP fused C terminally to CBDcenA + C-terminal linker driven by the IPTG inducible PLlac 0-1 promoter.
+
mRFP fused to the C terminal of CBDcenA + linker (BBa_K1615091) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the mRFP CBD fusion to express when induced with IPTG.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:59, 22 September 2015

CBDcenA+Linker fused to mRFP driven by LacI promoter

mRFP fused to the C terminal of CBDcenA + linker (BBa_K1615091) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the mRFP CBD fusion to express when induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]