Difference between revisions of "Part:BBa K1615099"

 
 
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<partinfo>BBa_K1615099 short</partinfo>
 
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mRFP fused C terminally to CBDcipA with N and C terminal linkers.
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mRFP (BBa_K1615089) fused to the C terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
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[[File:CBD RFP folding.jpg]]
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To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator. This picture shows that this biobrick does fold properly.
  
 
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Latest revision as of 00:56, 22 September 2015

Linker-CBDcipA-linker fused to mRFP in RFC25

mRFP (BBa_K1615089) fused to the C terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

CBD RFP folding.jpg

To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator. This picture shows that this biobrick does fold properly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]