Difference between revisions of "Part:BBa K1615111:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1615111 short</partinfo>
 
<partinfo>BBa_K1615111 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
RFC25 Compatible. E. coli optimised.
+
In an effort to improve the CBDCipA existing in the registry from iGEM14_Imperial we used the sequence from BBa_K1321014 and made it RFC25 compatible by removing the illegal EcoRI site at 148.
  
  

Latest revision as of 23:32, 21 September 2015

CBDcipA with N and C-terminal linker in RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In an effort to improve the CBDCipA existing in the registry from iGEM14_Imperial we used the sequence from BBa_K1321014 and made it RFC25 compatible by removing the illegal EcoRI site at 148.


Source

CBDcipA from Clostridium thermocellum

References