Difference between revisions of "Part:BBa K1639016:Design"
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===Source=== | ===Source=== | ||
− | + | It's a modified version of pTet-Off plasmid | |
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===References=== | ===References=== |
Latest revision as of 23:29, 21 September 2015
VP16 Transcriptional Activation Domain with miR223 and miR21 Binding Site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 349
Illegal PstI site found at 355 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 349
Illegal PstI site found at 355
Illegal NotI site found at 320 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 349
Illegal PstI site found at 355 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 349
Illegal PstI site found at 355
Illegal NgoMIV site found at 783 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 313
Design Notes
This part was designed without prefix and suffix. It's composed of small portion of tTA from pTet-off plasmid followed up by miR binding sites and SV40 polyA signal. We used this gene to express tTA protein but with miR binding sites. 1.Clone into pTet-off with Sal1 and Hind3 enzymes 2.Clone into pSB1C3 with appropiate enzymes located on vector
Source
It's a modified version of pTet-Off plasmid