Difference between revisions of "Part:BBa K1615089:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1615089 short</partinfo>
 
<partinfo>BBa_K1615089 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Codon optimised for E. coli. RFC 25 compatible.
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Codon optimised for E. coli and RFC 25 compatible.
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There is an existing RFC25 compatible RFP (BBa_K1351021) in the registry from iGEM14_LMU-Munich which was the basis for our sequence. We then added the RFC25 prefix without the Shine-Dalgarno sequence.
  
  

Latest revision as of 23:25, 21 September 2015

mRFP RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli and RFC 25 compatible. There is an existing RFC25 compatible RFP (BBa_K1351021) in the registry from iGEM14_LMU-Munich which was the basis for our sequence. We then added the RFC25 prefix without the Shine-Dalgarno sequence.


Source

mRFP highly engineered variant originally from Discosoma striata.

References