Difference between revisions of "Part:BBa K1615045"

Revision as of 22:51, 21 September 2015

Heroin Esterase in RFC25

Heroin esterase, an acetylmorphine carboxylesterase, was isolated from Rhodococcus erythropolis strain H1 in 1994 from the garden soil at Cambridge and is able to use heroin as its sole carbon and energy source by deacetylating the C-3 and C-6 groups to form morphine¹. The gene her encodes this enzyme and can be expressed in the chassis Escherichia coli². The pH optimum for this enzyme is pH8.5 in bicine buffer¹.



The activity of heroin esterase can be tested using 4-nitrophenyl acetate which is hydrolysed by heroin esterase to form 4-nitrophenol and acetate³. This produces a yellow colour which can be read at 410 nm.

The following table summarises the kinetic analysis and statistics of the measurement for heroin esterase.

The following table summarises the kinetic analyses and statistics of the measurement for heroin esterase fused to a cellulose binding domain.

The graph below shows the production of NADPH in the presence of heroin due to the activity of heroin esterase and morphine dehydrogenase.

¹Cameron, G. W., Jordan, K. N., Holt, P. J., Baker, P. B., Lowe, C. R., & Bruce, N. C. (1994). Identification of a heroin esterase in Rhodococcus sp. strain H1. Applied and environmental microbiology, 60(10), 3881-3883.

²Rathbone, D. A., Holt, P. J., Lowe, C. R., & Bruce, N. C. (1997). Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli. Applied and environmental microbiology, 63(5), 2062-2066.

³Sigma-aldrich. 4-nitrophenyl acetate product information.

Sequence and Features