Difference between revisions of "Part:BBa K1615022:Design"
 
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===Design Notes===
 
===Design Notes===
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Codon optimised for E. coli. RFC 25 compatible. N & C terminal portions of MaoA were ordered separately as gBlocks in RFC25 due to size constraints. Fusion was performed using RFC25.
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This monoamine oxidase A sequence was found in <i>Klebsiella pneumoniae</i><sup>3</sup> and was codon optimised for the chassis Escherichia coli as well as being made RFC25 compatible by adding the corresponding prefix and suffix sequences and removing illegal restriction sites.
  
  

Latest revision as of 22:22, 21 September 2015

Monoamine Oxidase A RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This monoamine oxidase A sequence was found in Klebsiella pneumoniae3 and was codon optimised for the chassis Escherichia coli as well as being made RFC25 compatible by adding the corresponding prefix and suffix sequences and removing illegal restriction sites.


Source

Monoamine Oxidase A from Klebsiella pneumoniae.

References