Difference between revisions of "Part:BBa K1615000:Design"

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===Design Notes===
 
===Design Notes===
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To make this gene standardised it was codon optimised for the chassis <i>Esherichia coli</i> as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.
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To make this gene standardised it was codon optimised for the chassis <i>Escherichia coli</i> as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.
  
  

Latest revision as of 22:18, 21 September 2015

Morphine-6-dehydrogenase RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make this gene standardised it was codon optimised for the chassis Escherichia coli as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.


Source

Genomic sequence from Pseudomonas putida.

References