Difference between revisions of "Part:BBa K1615000"

Revision as of 21:29, 21 September 2015

Morphine-6-dehydrogenase RFC25

The structural gene morphine-6-dehydrogenase (morA) was first isolated from Pseudomonas putida M10 as it is capable of growth with morphine as its sole carbon source¹. Morphine dehydrogenase catalyses the oxidation of both morphine and codeine to produce morphinone and codeinone respectively. During this process NADP⁺ is reduced to NADPH which means that this enzyme is frequently used to detect morphine and codeine².

To test the morphine dehydrogenase activity it can be coupled with morphine and NADP⁺ to produce morphinone and NADPH. The amount of NADPH produced can be measured at 340nm. File:Https://static.igem.org/mediawiki/2015/4/4f/Morphine dehydrogenase activity.jpeg

¹Bruce, N. C., Wilmot, C. J., Jordan, K. N., Trebilcock, A. E., Stephens, L. D. G., & Lowe, C. R. (1990). Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Archives of microbiology, 154(5), 465-470.
²Rathbone, D. A., Holt, P. J., Lowe, C. R., & Bruce, N. C. (1997). Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli. Applied and environmental microbiology, 63(5), 2062-2066.

Sequence and Features