Difference between revisions of "Part:BBa K1616009"
 
Line 2: Line 2:
 
<partinfo>BBa_K1616009 short</partinfo>
 
<partinfo>BBa_K1616009 short</partinfo>
  
−
The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane. It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)
+
 
 +
 
 +
<h3> <font style="color:#b22222">HokD toxin</font> </h3>
 +
<br>
 +
The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane.  
 +
It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)
 +
 
 +
<h3> <font style="color:#b22222">Reverse sequence</font> </h3>
 +
This composite part is composed of the reverse sequence of HokD with reverse RBS (<bbpart>BBa_K1616011</bbpart>) and the double terminator T7 reversed (<bbpart>BBa_B0025</bbpart>). This part has been created in order to work with a reversed promoter, which will allow the synthesized  of HokD toxin.
 +
 
 +
[[Image:Part toxin HokD reverse.png|center|600px|thumb|'''Fig. 1:'''  HokD reversed]]
 +
 
  
  
Line 20: Line 31:
  
 
===Design Notes===
 
===Design Notes===
−
The presence of illegal site have been checked.  
+
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.  
  
  
Line 29: Line 40:
  
 
===References===
 
===References===
 +
 
Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.
 
Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.

Latest revision as of 19:10, 21 September 2015

HokD reversed - RBS and double T7 terminator


HokD toxin


The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane. It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)

Reverse sequence

This composite part is composed of the reverse sequence of HokD with reverse RBS (BBa_K1616011) and the double terminator T7 reversed (BBa_B0025). This part has been created in order to work with a reversed promoter, which will allow the synthesized of HokD toxin.

Fig. 1: HokD reversed


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.


Source

This composition part is composed of reversed sequence of the HokD from BBa_K1616008, and RBS reversed BBa_K1616011 and the double terminator T7 reversed BBa_B0025.

References

Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.