Difference between revisions of "Part:BBa K1850001"
 
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===Usage and Biology===
 
===Usage and Biology===
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This part contains the ''fimH'' adhesin. The FimH protein is a subunit of a naturally occurring structure in some strains of ''E. coli'' called type 1 pili. These hairlike appendages typically manifest as organelles on the surface of pathogenic E. coli which are responsible for urinary tract infections in humans. The FimH adhesin is found at the tip of the pilus, and binds naturally to the sugar mannose. <partinfo>BBa_K1850000</partinfo> contains this part under control of a titratable rhamnose promoter, and can be cotransformed with <partinfo>BBa_K1850013</partinfo>, which contains the rest of the ''fim'' operon, and then induced to produce type 1 pili.
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This part contains the ''fimH'' adhesin. The FimH protein is a subunit of a naturally occurring structure in some strains of ''E. coli'' called type 1 pili. These hairlike appendages typically manifest as organelles on the surface of pathogenic E. coli which are responsible for urinary tract infections in humans. The FimH adhesin is found at the tip of the pilus, and binds naturally to the sugar mannose. <partinfo>BBa_K1850000</partinfo> contains this part under control of a titratable rhamnose promoter, and can be cotransformed with <partinfo>BBa_K1850013</partinfo>, which contains the rest of the ''fim'' operon, and induced to produce type 1 pili.
  
 
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Latest revision as of 17:35, 21 September 2015

fimH


Usage and Biology

This part contains the fimH adhesin. The FimH protein is a subunit of a naturally occurring structure in some strains of E. coli called type 1 pili. These hairlike appendages typically manifest as organelles on the surface of pathogenic E. coli which are responsible for urinary tract infections in humans. The FimH adhesin is found at the tip of the pilus, and binds naturally to the sugar mannose. BBa_K1850000 contains this part under control of a titratable rhamnose promoter, and can be cotransformed with BBa_K1850013, which contains the rest of the fim operon, and induced to produce type 1 pili.

FimH can be modified to bind to a variety of biotic and abiotic surfaces by introducing a binding peptide fusion with the desired affinity to site 225, 258 or the N terminal (recommended for folded binding motifs). These sites are highlighted in the following image orange, red and purple respectively:

Harvard_Fim_Animated.gif


Q5 PCR mutagenesis New England Biolabs was used for this purpose but other techniques may also be suitable. See our [http://2015.igem.org/Team:Harvard_BioDesign/Platform wiki] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]