Difference between revisions of "Repository:iGEM 2007 DNA distribution"

Revision as of 16:02, 19 April 2007

From Mid-April to Early May, the staff at the Registry prepared the Kit of Parts that will be sent to the 2007 iGEM teams.

Here is some useful information about the steps taken in that process.

Quality Control

Antibiotic resitance:

We tested the antibiotic resistance of each part sent out in the Kit of Parts by growing it in broth containing each of our 4 antibiotics. The test was done on a 384-deepwell plate filled with EZ Rich Defined Media which contained a cresol red indicator dye and the appropriate antibiotic. Each quadrant of the test plate contains one of the four antibiotics into which the part is inoculated (see layout below). After growing for 18hrs at 37C, images from each of the plates were collected. Since the broth contains a cresol red indicator that changes as the pH of the media decreases, you can tell whether the part grew by noting which of the wells turned from red to yellow since as the cells grow, the media becomes more acidic.

**384-deepwell test plate antibiotic quadrant layout:**
	1	2	3	4
A	Amp	Cm	Amp	Cm
B	Tet	Kan	Tet	Kan
C	Amp	Cm	Amp	Cm
D	Tet	Kan	Tet	Kan
...

Results for each source plate:

Revision as of 15:03, 19 April 2007 (view source) Meaganl (Talk \| contribs) ← Older edit		Revision as of 16:02, 19 April 2007 (view source) Meaganl (Talk \| contribs) Newer edit →
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−	We tested the antibiotic resistance of each part sent out in the Kit of Parts by growing it in broth containing each of our 4 antibiotics. The test was done on a 384-deepwell plate filled with EZ Rich Defined Media which contained a cresol red indicator dye and the appropriate antibiotic. Each quadrant of the test plate contains one of the four antibiotics into which the part is inoculated (see layout below). After growing for 18hrs at 37C, images from each of the plates were collected. Since the broth contains a cresol red indicator that changes as the pH of the media decreases, you can tell whether the part grew by noting which of the wells is yellow since as the cells grow, the media becomes more acidic.	+	We tested the antibiotic resistance of each part sent out in the Kit of Parts by growing it in broth containing each of our 4 antibiotics. The test was done on a 384-deepwell plate filled with EZ Rich Defined Media which contained a cresol red indicator dye and the appropriate antibiotic. Each quadrant of the test plate contains one of the four antibiotics into which the part is inoculated (see layout below). After growing for 18hrs at 37C, images from each of the plates were collected. Since the broth contains a cresol red indicator that changes as the pH of the media decreases, you can tell whether the part grew by noting which of the wells turned from red to yellow since as the cells grow, the media becomes more acidic.