Difference between revisions of "Part:BBa K1618024"

Revision as of 12:12, 21 September 2015

Pro-GlgB-Rv3034c

K1618024 contains the coding sequences for GlgB from E. coli and Rv3034c from Mycobacterium tuberculosis (strain ATCC 25618). GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3034c is a putative acetyltransferase. This part will potentially make branched, modified glycogen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 283
Illegal BamHI site found at 1491
Illegal BamHI site found at 2644
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

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 <partinfo>BBa_K1618024 short</partinfo>
-Rv3034c is a gene which encodes for a putative acyltransferase which will hopefully acylate glycogen chains. This gene is encoded alongside GlgB which codes for a branching enzyme which will cut alpha-1,4 links and re-anneal the strand to the main glycogen strand through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector. The bacteria therefore shows chloramphenicol resistance.
+K1618024 contains the coding sequences for GlgB from E. coli and Rv3034c from Mycobacterium tuberculosis (strain ATCC 25618). GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point.  Rv3034c is a putative acetyltransferase. This part will potentially make branched, modified glycogen.
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