Difference between revisions of "Part:BBa K1678006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.<br> | |
− | + | The T<sub>ldh</sub> terminator comes from L. buchneri lactate dehydrogenase gene. (also used in :'Spath et al. Microbial Cell Factories 2012, 11:141').<br> | |
− | + | The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'.<br> | |
+ | The whole cassette was artificially synthesized. | ||
===Source=== | ===Source=== | ||
===References=== | ===References=== |
Latest revision as of 11:54, 21 September 2015
mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 883
Design Notes
The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.
The Tldh terminator comes from L. buchneri lactate dehydrogenase gene. (also used in :'Spath et al. Microbial Cell Factories 2012, 11:141').
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'.
The whole cassette was artificially synthesized.