Difference between revisions of "Part:BBa K1846007:Design"

Latest revision as of 01:02, 21 September 2015

Bacteriophage lambda tail fibre assembly (tfa) under TetR regulation

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We have combined the P(Cat)+TetR circuit BBa_K1846003 and the tfa circuit BBa_K1846001 into a composite part using a 3A assembly. The cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This composite part has been registered as BBa_K1846007.

Source

2015 iGEM distribution kit and DNA synthesis

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This cI-Cro circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by DNA agarose electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part [https://parts.igem.org/Part:BBa_K1846007 BBa_K1846007].
+We have combined the P(Cat)+TetR circuit [https://parts.igem.org/Part:BBa_K1846003 BBa_K1846003] and the tfa circuit [https://parts.igem.org/Part:BBa_K1846001 BBa_K1846001] into a composite part using a 3A assembly. The cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This composite part has been registered as BBa_K1846007.
-[[File:BBKiGEM-cICro-circuit.jpg|400px|thumb|none|]]
+[[File:BBKiGEM-P(Cat)+TetR+tfa-circuit.jpg|400px|thumb|none|]]
 ===Source===
-DNA synthesis
+iGEM distribution kit and DNA synthesis
 ===References===