Difference between revisions of "Part:BBa K1846005:Design"
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<partinfo>BBa_K1846005 short</partinfo> | <partinfo>BBa_K1846005 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | This cI-Cro circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by DNA agarose electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part [https://parts.igem.org/Part:BBa_K1846007 BBa_K1846007]. | ||
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| + | [[File:BBKiGEM-cICro-circuit.jpg|400px|thumb|none|]] | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 00:35, 21 September 2015
cI-Cro bacteriophage lambda lytic cycle regulatory circuit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This cI-Cro circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by DNA agarose electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part BBa_K1846007.
Source
DNA synthesis