Difference between revisions of "Part:BBa K1846003:Design"
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TetR generator cassette [https://parts.igem.org/Part:BBa_P0140 BBa_P0140] and P(Cat) promoter [https://parts.igem.org/Part:BBa_I14033 BBa_I14033] were obtained from 2015 iGEM distribution kit.  and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part [https://parts.igem.org/Part:BBa_K1846003 BBa_K1846003].
 
TetR generator cassette [https://parts.igem.org/Part:BBa_P0140 BBa_P0140] and P(Cat) promoter [https://parts.igem.org/Part:BBa_I14033 BBa_I14033] were obtained from 2015 iGEM distribution kit.  and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part [https://parts.igem.org/Part:BBa_K1846003 BBa_K1846003].
  
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Revision as of 23:26, 20 September 2015

TetR circuit with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TetR generator cassette BBa_P0140 and P(Cat) promoter BBa_I14033 were obtained from 2015 iGEM distribution kit. and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part BBa_K1846003.

BBKiGEM-Pcat-TetR.jpg


Source

2015 iGEM distribution kit


References