Difference between revisions of "Part:BBa K1846003:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | TetR generator cassette [https://parts.igem.org/Part:BBa_P0140 BBa_P0140] and P(Cat) promoter [https://parts.igem.org/Part:BBa_I14033 BBa_I14033] were obtained from 2015 iGEM distribution kit. and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part [https://parts.igem.org/Part:BBa_K1846003 BBa_K1846003]. | ||
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| + | <img alt="File:BBKiGEM-ORF-314.jpg" src="/wiki/images/thumb/3/3d/BBKiGEM-ORF-314.jpg/265px-BBKiGEM-ORF-314.jpg" height="599" width="265"> | ||
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| + | [[File:BBKiGEM-Pcat-TetR.jpg]] | ||
===Source=== | ===Source=== | ||
| − | + | 2015 iGEM distribution kit | |
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===References=== | ===References=== | ||
Revision as of 22:43, 20 September 2015
TetR circuit with constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
TetR generator cassette BBa_P0140 and P(Cat) promoter BBa_I14033 were obtained from 2015 iGEM distribution kit. and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part BBa_K1846003.
Source
2015 iGEM distribution kit