Difference between revisions of "Part:BBa K1846008:Design"
 
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The sequence derives from the bacteriophage lambda genomic sequence, and was adapted from Technion's 2012 BioBrick [https://parts.igem.org/Part:BBa_K784017 BBa_K784017].
 
The sequence derives from the bacteriophage lambda genomic sequence, and was adapted from Technion's 2012 BioBrick [https://parts.igem.org/Part:BBa_K784017 BBa_K784017].
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===References===
 
===References===

Latest revision as of 22:28, 20 September 2015

Bacteriophage lambda tip attachment protein J


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
    Illegal AgeI site found at 1471
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The J gene was amplified from Technion's 2012 BioBrick BBa_K784017. The sequence contains a number of illegal PstI restriction sites. We haven't been able to complete the removal of the restriction sites in time; so the part has not as yet been submitted to the registry.


Source

The sequence derives from the bacteriophage lambda genomic sequence, and was adapted from Technion's 2012 BioBrick BBa_K784017.


References