Difference between revisions of "Part:BBa K1846008:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1846008 short</partinfo> | <partinfo>BBa_K1846008 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | The J gene was amplified from Technion's 2012 BioBrick [https://parts.igem.org/Part:BBa_K784017 BBa_K784017]. The sequence contains a number of illegal PstI restriction sites. We haven't been able to complete the removal of the restriction sites in time; so the part has not as yet been submitted to the registry. | ||
Revision as of 22:27, 20 September 2015
Bacteriophage lambda tip attachment protein J
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 577
Illegal PstI site found at 727
Illegal PstI site found at 1886 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 577
Illegal PstI site found at 727
Illegal PstI site found at 1886 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 577
Illegal PstI site found at 727
Illegal PstI site found at 1886 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 577
Illegal PstI site found at 727
Illegal PstI site found at 1886
Illegal AgeI site found at 1471 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The J gene was amplified from Technion's 2012 BioBrick BBa_K784017. The sequence contains a number of illegal PstI restriction sites. We haven't been able to complete the removal of the restriction sites in time; so the part has not as yet been submitted to the registry.
Source
The sequence derives from the bacteriophage lambda genomic sequence, and was adapted from Technion's 2012 BioBrick BBa_K784017.