Difference between revisions of "Part:BBa K1846008"
 
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The bacteriophage lambda ''J'' gene encodes the tip attachment protein, responsible for host protein recognition and specificity [1]. C-terminal residues of protein J have direct binding interactions with the maltoporin (LamB), a membrane protein  of ''E.coli'', allowing irreversible attachment of the phage to the host and facilitating DNA injection [1],[2].
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The bacteriophage lambda ''J'' gene encodes the tip attachment protein that forms the base plate of the bacteriophage's tail. It is responsible for host protein recognition and specificity. C-terminal residues of protein J have direct binding interactions with the maltoporin (LamB), a membrane protein  of ''E.coli'', allowing irreversible attachment of the phage to the host and facilitating DNA injection [1],[2].
  
  

Latest revision as of 22:23, 20 September 2015

Bacteriophage lambda tip attachment protein J

Sequence for the bacteriophage lambda J gene.


Usage and Biology

The bacteriophage lambda J gene encodes the tip attachment protein that forms the base plate of the bacteriophage's tail. It is responsible for host protein recognition and specificity. C-terminal residues of protein J have direct binding interactions with the maltoporin (LamB), a membrane protein of E.coli, allowing irreversible attachment of the phage to the host and facilitating DNA injection [1],[2].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 577
    Illegal PstI site found at 727
    Illegal PstI site found at 1886
    Illegal AgeI site found at 1471
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Chatterjee, S., & Rothenberg, E. (2012). Interaction of bacteriophage λ with its E. coli receptor, LamB. Viruses, 4(11), 3162–3178. doi:10.3390/v4113162

[2] Werts, C., Michel, V., Hofnung, M., & Charbit, A. (1994). Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range. Journal of Bacteriology, 176(4), 941–947.