Difference between revisions of "Part:BBa K1846001:Design"

 
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<partinfo>BBa_K1846001 short</partinfo>
 
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<partinfo>BBa_K1846001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1846001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by DNA agarose electrophoresis (Figure 1). This BioBrick was registered as part [https://parts.igem.org/Part:BBa_K1846001 BBa_K1846001].
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BBKiGEM-tfa-circuit.jpg
  
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<img alt="File:BBKiGEM-ORF-314.jpg" src="/wiki/images/thumb/3/3d/BBKiGEM-ORF-314.jpg/265px-BBKiGEM-ORF-314.jpg" height="599" width="265">
  
 
===Source===
 
===Source===
  
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DNA synthesis
  
 
===References===
 
===References===

Revision as of 21:40, 20 September 2015

Bacteriophage lambda tail fibre assembly protein circuit



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by DNA agarose electrophoresis (Figure 1). This BioBrick was registered as part BBa_K1846001.




BBKiGEM-tfa-circuit.jpg

<img alt="File:BBKiGEM-ORF-314.jpg" src="/wiki/images/thumb/3/3d/BBKiGEM-ORF-314.jpg/265px-BBKiGEM-ORF-314.jpg" height="599" width="265">

Source

DNA synthesis

References