Difference between revisions of "Part:BBa I721002:Experience"

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<h2>Team Bielefeld-CeBiTec 2015</h2>
 
<h2>Team Bielefeld-CeBiTec 2015</h2>
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We used a modified version of team Brown 2007´s lead biosensor. We used an <i>E. coli</i> codon optimzed version of the repressor <a href"https://parts.igem.org/Part:BBa_K1758330">BBa_K1758330</a> and combined it with their lead responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP <a href"https://parts.igem.org/Part:BBa_K1758332">BBa_K1758332</a>. Using this two we could characterize the lead sensor <i>in vitro</i> in our <a href"http://2015.igem.org/Team:Bielefeld-CeBiTec/Project/CFPS">CFPS</a> system.
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We used a modified version of team Brown 2007´s lead biosensor. We used an <i>E. coli</i> codon optimzed version of the repressor <a href"https://parts.igem.org/Part:BBa_K1758330">BBa_K1758330</a> and combined it with their lead responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP <a href"https://parts.igem.org/Part:BBa_K1758332">BBa_K1758332</a>. Using this two we could creat a device for lead detection <a href"https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>.
  
 
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Latest revision as of 21:08, 20 September 2015


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Team Bielefeld-CeBiTec 2015

We used a modified version of team Brown 2007´s lead biosensor. We used an E. coli codon optimzed version of the repressor BBa_K1758330 and combined it with their lead responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP BBa_K1758332. Using this two we could creat a device for lead detection BBa_K1758333.