Difference between revisions of "Part:BBa K1614022"
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MAWS software generated aptamer for the tetramerization domain of p53 linked with a 5x A-Linker to the HRP-mimicking DNAzyme. | MAWS software generated aptamer for the tetramerization domain of p53 linked with a 5x A-Linker to the HRP-mimicking DNAzyme. | ||
| − | This part comprises an aptamer designed to targed | + | This part comprises an aptamer designed to targed p53 using <b>MAWS</b> a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5. |
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Latest revision as of 19:36, 20 September 2015
p53 AptaBody
MAWS software generated aptamer for the tetramerization domain of p53 linked with a 5x A-Linker to the HRP-mimicking DNAzyme.
This part comprises an aptamer designed to targed p53 using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]