Difference between revisions of "Part:BBa K1614023"
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<partinfo>BBa_K1614023 short</partinfo> | <partinfo>BBa_K1614023 short</partinfo> | ||
| − | MAWS software generated actin aptamer (8mer) linked with a 5x A linker to the horseradish peroxidase | + | MAWS software generated actin aptamer (8mer) linked with a 5x A linker to the horseradish peroxidase mimic DNAzyme. |
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| + | This part comprises an aptamer designed to targed actin using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:34, 20 September 2015
Actin AptaBody with software generated actin aptamer (8mer)
MAWS software generated actin aptamer (8mer) linked with a 5x A linker to the horseradish peroxidase mimic DNAzyme.
This part comprises an aptamer designed to targed actin using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]